Exocytosis in Drosophila
نویسندگان
چکیده
Antibody Production The cDNA sequence corresponding to amino acids 1-416 of Vha100-1 was amplified by PCR to incorporate BamHI and NotI restriction sites and then cloned into to the pET28a (Invitrogen) vector for protein expression. The primers used were: 5’-CGGAATTCATGGGTTCCCTATTCCGCAGCGAGG-3’ and 5’-CCGCTCGAGGGATTCATCTCCCGATAGCTGGCC-3’. Guinea pig antibodies against this domain were raised by Cocalico Biologicals (Reamstown, PA) using the purified recombinant protein.
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